If you need to use tracking numbers, rather than order number, to cue an order in GroovePacker, you'd check this option.
Settings > System Settings >Scan & Pack Settings
There you'll see "Cue Orders For Scan & Pack By Tracking Number"
You'd need to ensure that tracking number is being imported with order, and it's being saved. The barcode will need to include the tracking number that's coming in. It doesn't have to be an exact match - as long as it's included in the barcode. If there are any issues with this, contact support and we can help you work through it.